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中英文說明丨艾美捷血管緊張素ⅡELISA試劑盒方案

2023-01-17 15:17 作者:艾美捷  | 我要投稿

Angiotensins are small peptides derived from angiotensinogen. Several of the known angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I (DRVYIHPFHL) has no known effector function but it is an immediate precursor of Angiotensin II (DRVYIHPF). Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg-vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A (ARVYIHPF), and Angiotensin III (RVYIHPF), also bind AT1 receptors. Angiotensin (1-7) (DRVYIHP) binds a different receptor called MAS-1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV (VYIHPF) binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVV-hemorphin-7 (LVVYPWTQRF), which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that have been identified, including Ang (1-9) (DRVYIHPFH), Ang (1-12) (DRVYIHPFHLVI), Ang V(3-7) (VYIHP), as well as several other shorter peptides that have undetermined functions.

Sensitivity:?4.6 pg/ml (range 3.9-10,000 pg/ml)

Assay Time:?3.5 hours

Applications:?ELISA, Colorimetric detection

Application Notes:?For the quantitative determination of species independent Angiotensin II in plasma and serum samples.

Wavelength:?450 nm

Species reactivity:?Species independent

Crossreactivity:?Angiotensin II (100%), Angiotensin A (100%), Angiotensin III (100%), Angiotensin IV (100%), Angiotensin I (0.319%), Angiotensin (1-9) (0.103%), LVV-hemorphin 7 (0.067%), Angiotensin (1-7) (0.053%), Bradykinin (0.022%)

Quantity:?1 x 96-well plate

Use/Stability:?Store all components at +4oC, except Standard at -20oC.

Shipping:?Shipped on Blue Ice

Contents:?Microtiter Plate, Conjugate, Antibody, Assay Buffer 16, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Streptavidin-HRP

?

ADI-900-204血管緊張素ⅡELISA試劑盒:高靈敏度ELISA,檢測(cè)量低至4.6pg/ml。

艾美捷ADI-900-204?血管緊張素ⅡELISA試劑盒化學(xué)性質(zhì):

靈敏度:4.6 pg/ml(范圍3.9-10000 pg/ml)

化驗(yàn)時(shí)間:3.5小時(shí)

應(yīng)用:ELISA,比色檢測(cè)

應(yīng)用說明:用于定量測(cè)定血漿和血清樣品中的種類無關(guān)的血管緊張素II。

波長(zhǎng):450 nm

物種反應(yīng)性:物種無關(guān)

交叉反應(yīng)性:血管緊張素II(100%),血管緊張素A(100%)、血管緊張素III(100%)和血管緊張素IV(100%)

數(shù)量:1 x 96孔板

使用/穩(wěn)定性:將所有組件儲(chǔ)存在+4oC,標(biāo)準(zhǔn)組件除外,儲(chǔ)存在-20oC。

運(yùn)輸:藍(lán)冰運(yùn)輸

內(nèi)容:微量滴定板、綴合物、抗體、測(cè)定緩沖液16、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、TMB底物、停止溶液2、鏈霉親和素HRP

?

血管緊張素ⅡELISA試劑盒文獻(xiàn)參考:

Hypertension enhances advanced atherosclerosis and induces cardiac death in Watanabe heritable hyperlipidemic rabbits: B. Ning, et al.; Am. J. Pathol. 188, 2936 (2018);

Post-translational modifications of eNOS augment nitric oxide availability and facilitates hypoxia adaptation in Ladakhi women: Pooja, et al.; Nitric Oxide 78, 103 (2018), Application(s): ELISA using human plasma;

Prorenin independently causes hypertension and renal and cardiac fibrosis in cyp1a1-prorenin transgenic rats: G. Zhou, et al.; Clin. Sci. (Lond.) 132, 1345 (2018);

12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of Angiotensin levels in rat;


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