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百螢 核酸檢測的好幫手----DNA檢測染料 PUR-1

2023-11-24 10:27 作者:百螢生物  | 我要投稿

一、百螢 DNA檢測染料 PUR-1簡介

? 在沒有核酸的情況下,PUR-1基本不發(fā)熒光。在存在DNA或RNA的情況下,其熒光顯著增強。在存在RNA的情況下,PUR-1在?478 nm處具有熒光,熒光量子產(chǎn)率為?40%。PUR-1染料在488 nm處可激發(fā)(在460 nm處有大激發(fā)),并且在存在核酸的情況下發(fā)出470至550 nm之間的熒光,在478 nm處具有大發(fā)射。該染料可與流式細胞儀結(jié)合用于分析血源性寄生蟲感染。 ??

二、百螢 DNA檢測染料 PUR-1參數(shù)

?分子量?? ??437.30 溶??劑?? ?DMSO 存儲條件?? 在-15℃以下保存,避光防潮 保質(zhì)期??? ?12個月 激發(fā)波長(nm) ?459 發(fā)射波長(nm)?? 478 ?

三、百螢 DNA檢測染料 PUR-1參考文獻

? Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria

Authors:

?Slocinska M, Rosinski G, Jarmuszkiewicz W.

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?Protein Pept Lett?(2016):?63 ? Rasagiline prevents cyclosporine A-sensitive superoxide flashes induced by PK11195, the initial signal of mitochondrial membrane permeabilization and apoptosis

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?Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.

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?J Neural Transm (Vienna)?(2016):?491 ? Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1

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?Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.

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?Biochim Biophys Acta?(2016):?1102 ? Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model

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Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.

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?Int J Mol Sci?(2016):?1293 ? Manganese-superoxide dismutase (Mn-SOD) overexpression is a common event in colorectal cancers with mitochondrial microsatellite instability

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?Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.

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?Tumour Biol.?(2016) ? Production of superoxide/hydrogen peroxide by the mitochondrial 2-oxoadipate dehydrogenase complex

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?Goncalves RL, Bunik VI, Br and MD., undefined

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?Free Radic Biol Med?(2016):?247 ? A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury

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?Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.

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Cell Metab?(2016):?254 ? Rotenone-stimulated superoxide release from mitochondrial complex I acutely augments L-type Ca2+ current in A7r5 aortic smooth muscle cells

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Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.

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?Am J Physiol Heart Circ Physiol?(2016):?H1118 ? Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity

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?Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.

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J Cell Biochem?(2016):?1352 ? Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy

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?Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.

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?Diabetes?(2016):?255

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