質譜應用教程-如何調高 QTof 的分辨率
目的
增加 QTof 的質譜分辨率。
環(huán)境
MassLynx 4.1
Synapt G2-Si
Synapt G2-S
Synapt G2
Xevo G2 XS
Xevo GS S
Xevo G2
Xevo QTof
步驟
在 Tune 頁面上，從設置下拉菜單中選擇 System View（系統(tǒng)視圖）。密碼：access。
選擇 Acquisition Settings（采集設定）。您將在左下角看到分辨率。這是此模式的預期分辨率。記下該分辨率并退出此窗口。
放大鎖定質量數(shù)化合物的峰。
通過降低 LockSpray 毛細管電壓，將峰計數(shù)調整為中等至低 e4 計數(shù)?；蛘哌M入 pDRE Test（pDRE 測試）下的 System 2（系統(tǒng) 2）選項卡，選擇 Attenuate（衰減）并調整 Transmission（傳輸），直到調整為低 e4 計數(shù)。注意：如果您愿意，請右鍵單擊 Tune 頁面中的峰，然后選擇 Intensity（強度）> 選擇 Normalize Data（歸一化數(shù)據(jù)）。這樣可以在調節(jié)分辨率時查看強度是否增加或降低。
放大峰，以便輕松查看峰形。
打開 System 2（系統(tǒng) 2）頁面，使用向左或向右箭頭鍵調節(jié)反射器網(wǎng)格reflectron grid電壓（單擊黑色數(shù)字右側的滑塊，然后使用鍵盤上的箭頭鍵進行調節(jié)）。調節(jié)以獲取最佳分辨率、峰形和靈敏度，分辨率優(yōu)先級最高。
接下來打開 System 1（系統(tǒng) 1）頁面，使用向左或向右箭頭鍵調節(jié)推動pusher offset電壓偏置值（單擊黑色數(shù)字右側的滑塊，然后使用鍵盤上的箭頭鍵進行調節(jié)）。調節(jié)獲取最佳分辨率、峰形和靈敏度，對稱峰形優(yōu)先級最高。
How to tune up the resolution on a QTof - WKB13440
Last updatedDec 21, 2021
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Article number: 13440
OBJECTIVE or GOAL
Increase the mass spectral resolution on a QTof.
ENVIRONMENT
MassLynx 4.1
SYNAPT G2 Si
SYNAPT G2 S
SYNAPT G2
Xevo G2 XS
Xevo GS S
Xevo G2
Xevo QTof
PROCEDURE
On the Tune page, from the setup drop-down menu, select System View. Password: "access".
Select Acquisition Settings. On the bottom left, you will see Resolution. This is the expected resolution for this mode. Take note of the resolution and exit this window.
Zoom in on lockmass peak.
Adjust counts to mid to low e4 counts by dropping the LockSpray capillary voltage. Or going to the System 2 tab under pDRE Test, select Attenuate, and adjust the Transmission until you have low e4 counts. Note: If you are comfortable doing so, right-click the peak in the tune page and select Intensity > select Normalize Data. This allows you to see if the intensity is going up or down as you tune the resolution.
Zoom in on the peak so that you can easily see the peak shape.
Go to the System 2 page and adjust the reflectron grid by using the left or right arrow key (click on the slider to the right of the number in black, and then use the arrow keys on the keyboard). Tune for best resolution, peak shape, and sensitivity, with resolution being the highest priority.
Now go to the System 1 page and adjust the pusher offset by using the left or right arrow key (click on the slider to the right of the number in black, and then use the arrow keys on the keyboard). Tune for best resolution, peak shapem and sensitivity, with symmetrical peak shape being the highest priority.
